Wesleyan portrait of Donald B. Oliver

Donald B. Oliver

Daniel Ayres Professor of Biology

860-685-3556

Professor of Molecular Biology and Biochemistry

Shanklin Laboratory, 210A
860-685-3556

Professor, Integrative Sciences

doliver@wesleyan.edu

BS Brandeis University
MAA Wesleyan University
PHD Tufts University

Donald B. Oliver

We are using the simple, well-characterized, and genetically facile bacterium E. coli as a model system to study the molecular details of protein translocation across the plasma membrane. Our major focus is on SecA ATPase, a DEAD motor protein that binds preproteins and the SecYEG channel complex, and which undergoes ATP-driven conformational cycles at the membrane that drive the stepwise translocation of proteins across the membrane. Genetic, biochemical and biophysical approaches are being utilized along with recent X-ray structures of SecA and SecYEG proteins in order to elucidate a number of important questions in this system: 1. SecA ATPase enzymology. SecA is a multi-domain protein whose conformational cycling is regulated by binding nucleotide, preproteins, SecYEG, and acidic phospholipids. SecA mutant proteins are being generated and utilized to define the enzymology of this complex ATPase. 2. SecA association with preproteins and SecYEG. Multidisciplinary approaches are being utilized to define the structural details of SecA interaction with signal peptides, preproteins, and SecYEG and to elucidate their functional consequences in order to work out the protein translocation cycle. 3. SecA dimer function. The nature of the requirement for SecA homo-dimerization in protein translocation is being elucidated.

Academic Affiliations

Office Hours

Monday 3-5 PM

Wednesday 2-4 PM

Courses

Spring 2018
MB&B 232 - 01
Immunology

MB&B 586 - 01
Seminar in Molecular Biology