Scientific Imaging Solutions

At Wesleyan, we offer cutting-edge scientific imaging solutions to advance your research projects. Our state-of-the-art equipment includes the Leica SP8 conformal microscope and the HITACHI SU5000 scanning electron microscope (with energy-dispersible x-ray spectroscopy).

Our focuses are on biological and non-biological material analysis. At Wesleyan we serve a wide range of researchers from several departments, including; Biology, Molecular Biology and Biochemistry, Chemistry, Molecular BioPhysics, Earth and Environmental Sciences and others.

Leica SP8 Confocal Microscope:

Super resolution high speed imaging
Provides exceptional imaging capabilities, allowing you to explore biological materials with high-resolution, 3D visualization
Simultaneous multicolor imaging with true super-resolution (as fine as 120nm)
Versatile features enable precise fluorescence and spectral imaging, making it ideal for a wide range of applications
Leica's exclusive detection concept of LIGHTNING allows you to trace the dynamics of multiple molecules, even those expressed at low levels, simultaneously over long recording times in living specimens

Specifications:

DMi8 inverted microscope stand (DMi8CEL)
- Super Z Galvo Stage; optional Universal or Multiwell plate inserts
- Transmitted light PMT
- Blue/green/red/far red filter cubes for wide-field fluorescence

Lasers (two diode and 3 optically pumped semiconductor laser)
- Diode 405
- Diode 638
- OPSL 488
- OPSL 514
- OPSL 552

Objectives:
- 5x (HC PL FLUOTAR 5x/0.15 DRY)
  - Free working distance: 13.700mm
- 10x (HC PL APO CS2 10x/0.40 DRY)
  - Free working distance: 2.740mm
- 20x (HC PL APO CS2 20x/0.75 DRY)
  - Free working distance: 0.620mm
- 25x (water) (Fluotar VISIR 25x/0.95 WATER)
  - Free working distance: 2.400mm
- 40x (oil) (HC PL APO CS2 40x/1.30 OIL)
  - Free working distance: 0.240mm
- 63x (oil) (HC PL APO CS2 63x/1.40 OIL)
  - Free working distance: 0.140mm

TCS SP8 Confocal Scan Head
- Non-resonant (conventional/variable) Scanner: 1 – 1800 Hz (7 fps @ 512 x 512 px, 84 fps @ 512 x 16 px), line frequency up to 3600 lines/second (bidirectional), max. scan format 8192 x 8192 px, scan field 22 mm diag.
- Resonant Scanner (ultra-fast, reduced photo damage): 8000 Hz (28 fps @ 512 x 512 px, 290 fps @ 512 x 16 px), line frequency up to 16,000 lines/second (bidirectional), max. scan format 2496 x 2496 px, scan field 13 mm diag. Optical field rotation 200°.

Detectors: Lambda-Scan mode permits recording of spectral image series
- 2 HyD hybrid Spectral detectors:
  - Very low dark noise
  - Photon counting capability
  - Enables time gated detection
- 1 PMTs:
  - 40 MHz sampling
  - Detection range 400–800 nm
- Transmitted light detector (TLD)
  - Ideal for bright image acquisition

HITACHI SU5000 Scanning Electron Microscope (SEM):

Ideal for characterizing surface structures, particles, and materials with utmost precision
Suitable for conductive and non-conductive materials
Ideal for variable pressure, allowing for high and low voltage image analysis
Super high-resolution imaging at a nano scale level

Types of Detectors:

Dual secondary electron (SE) detector

Enhanced image quality at lower voltages and any working distance

Back Scatter detector (BSD)

Retractable and angular detector provides high quality signal mixing. Choose between selective low energy or high energy back scatter electron signal collection