Scientific Imaging Solutions
At Wesleyan, we offer cutting-edge scientific imaging solutions to advance your research projects. Our state-of-the-art equipment includes the Leica SP8 conformal microscope and the HITACHI SU5000 scanning electron microscope (with energy-dispersible x-ray spectroscopy).
Our focuses are on biological and non-biological material analysis. At Wesleyan we serve a wide range of researchers from several departments, including; Biology, Molecular Biology and Biochemistry, Chemistry, Molecular BioPhysics, Earth and Environmental Sciences and others.
Leica SP8 Confocal Microscope:
- Super resolution high speed imaging
- Provides exceptional imaging capabilities, allowing you to explore biological materials with high-resolution, 3D visualization
- Simultaneous multicolor imaging with true super-resolution (as fine as 120nm)
- Versatile features enable precise fluorescence and spectral imaging, making it ideal for a wide range of applications
- Leica's exclusive detection concept of LIGHTNING allows you to trace the dynamics of multiple molecules, even those expressed at low levels, simultaneously over long recording times in living specimens
Specifications:
- DMi8 inverted microscope stand (DMi8CEL)
- Super Z Galvo Stage; optional Universal or Multiwell plate inserts
- Transmitted light PMT
- Blue/green/red/far red filter cubes for wide-field fluorescence
- Lasers (two diode and 3 optically pumped semiconductor laser)
- Diode 405
- Diode 638
- OPSL 488
- OPSL 514
- OPSL 552
- Objectives:
- 5x (HC PL FLUOTAR 5x/0.15 DRY)
- Free working distance: 13.700mm
- 10x (HC PL APO CS2 10x/0.40 DRY)
- Free working distance: 2.740mm
- 20x (HC PL APO CS2 20x/0.75 DRY)
- Free working distance: 0.620mm
- 25x (water) (Fluotar VISIR 25x/0.95 WATER)
- Free working distance: 2.400mm
- 40x (oil) (HC PL APO CS2 40x/1.30 OIL)
- Free working distance: 0.240mm
- 63x (oil) (HC PL APO CS2 63x/1.40 OIL)
- Free working distance: 0.140mm
- 5x (HC PL FLUOTAR 5x/0.15 DRY)
- TCS SP8 Confocal Scan Head
- Non-resonant (conventional/variable) Scanner: 1 – 1800 Hz (7 fps @ 512 x 512 px, 84 fps @ 512 x 16 px), line frequency up to 3600 lines/second (bidirectional), max. scan format 8192 x 8192 px, scan field 22 mm diag.
- Resonant Scanner (ultra-fast, reduced photo damage): 8000 Hz (28 fps @ 512 x 512 px, 290 fps @ 512 x 16 px), line frequency up to 16,000 lines/second (bidirectional), max. scan format 2496 x 2496 px, scan field 13 mm diag. Optical field rotation 200°.
- Detectors: Lambda-Scan mode permits recording of spectral image series
- 2 HyD hybrid Spectral detectors:
- Very low dark noise
- Photon counting capability
- Enables time gated detection
- 1 PMTs:
- 40 MHz sampling
- Detection range 400–800 nm
- Transmitted light detector (TLD)
- Ideal for bright image acquisition
- 2 HyD hybrid Spectral detectors:
HITACHI SU5000 Scanning Electron Microscope (SEM):
- Ideal for characterizing surface structures, particles, and materials with utmost precision
- Suitable for conductive and non-conductive materials
- Ideal for variable pressure, allowing for high and low voltage image analysis
- Super high-resolution imaging at a nano scale level
Types of Detectors:
- Dual secondary electron (SE) detector
- Back Scatter detector (BSD)
- Ultra-variable Pressure Detector (UVD)
- Energy Dispersible X-ray Spectroscopy (EDS)