SCIE 680
Molecular Biology Laboratory
Michael McAlear
Course Schedule | |
June 18 | Check in, introductions
Lecture: Basics of molecular biology Exp 1: Run a digest of plasmid DNA Exp 2: Dilute, plate, irradiate yeast |
June 19 | Lecture: The Polymerase Chain Reaction
Exp 3: Do PCR reaction, run on gel Lecture: DNA sequencing |
June 20 | Exp 1: Induce E. coli with IPTG Collect cells Exp 4: Inoculate yeast onto plates |
June 21 | Lecture: Protein purification
Exp 1: Lyse E. coli cells Lecture: Protein synthesis |
June 22 | Exp 1: Get de-stained protein gel Exp 2: Count viable yeast colonies, plot viabilities Exp 4: Score yeast plates for growth Lecture: DNA microchips |
June 23 | Wrap up |